In EFL League Two, a club can have a squad of up to 22 players. This limit doesn't include goalkeepers, and it also doesn't include players under the age of 21 at the start of the calendar year.
Here's a more detailed breakdown:
Maximum Squad Size: 22 players.
Goalkeepers: Don't count towards the 22-player limit.
Under-21 Players: Players who are under 21 at the start of the calendar year are not included in the 22-player limit.
Home-Grown Players: At least 8 of the 22 registered players must be home-grown.